Engineering thermostability in serine protease inhibitors
نویسندگان
چکیده
منابع مشابه
Engineering thermostability in serine protease inhibitors.
Unlike most globular proteins, the native form of serine protease inhibitors (serpins) is strained. Previous studies of human alpha(1)-antitrypsin, a prototype plasma serpin, revealed that various unfavorable interactions, such as overpacking of side chains, buried polar groups and cavities, are the structural basis of the strain. The local strain could be relieved by various stabilizing single...
متن کاملEffect of different serine protease inhibitors in validating the 115 kDa Leishmania donovani secretory serine protease as chemotherapeutic target.
Proteases have been considered as an important group of targets for development of antiprotozoal drugs due to their essential roles in host-parasite interactions, parasite immune evasion, life cycle transition and pathogenesis of parasitic diseases. The development of potent and selective serine protease inhibitors targeting L. donovani secretory serine protease (pSP) could pave the way to the ...
متن کاملDiverse Clinical Functions of Serine Protease and Its Inhibitors
Serine protease performs normal biological functions in cell. Serine protease are involves in coagulation, inflammation and immunity. Wide variety organisms produce serine proteases including virus, bacteria, animals and plants. Serine proteases are mono-meric enzymes. Serine protease are produced as inactive enzymes called zymogen and transported to other organs where they needed, which are th...
متن کاملTailored Ahp-cyclodepsipeptides as Potent Non-covalent Serine Protease Inhibitors.
The S1 serine protease family is one of the largest and most biologically important protease families. Despite their biomedical significance, generic approaches to generate potent, class-specific, bioactive non-covalent inhibitors for these enzymes are still limited. In this work, we demonstrate that Ahp-cyclodepsipeptides represent a suitable scaffold for generating target-tailored inhibitors ...
متن کاملSchistosome serine protease inhibitors: parasite defense or homeostasis?
Serpins are a structurally conserved family of macromolecular inhibitors found in numerous biological systems. The completion and annotation of the genomes of Schistosoma mansoni and Schistosoma japonicum has enabled the identification by phylogenetic analysis of two major serpin clades. S. mansoni shows a greater multiplicity of serpin genes, perhaps reflecting adaptation to infection of a hum...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2004
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzh036